Bacteria and biochar boost hemp yields in salty soil

Phosphorus-solubilizing bacteria and phosphorus-enriched biochar enhance growth, cannabinoid content, and essential oil yield in salt-stressed hemp (Cannabis sativa L.).

Plant science : an international journal of experimental plant biology • • Moderately Relevant
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AI Summary

This research explores how combining phosphorus-solubilizing bacteria (PSB) with phosphorus-enriched biochar can help hemp plants thrive under saline soil conditions. Salt stress typically harms cannabis crops by creating phosphorus deficiencies, which reduces plant size, health, and cannabinoid production. The study tested two bacterial strains (Bacillus subtilis and Pseudomonas putida) alongside biochar amendments in hemp plants exposed to salt stress equivalent to severely degraded soils.

The combined treatment dramatically improved plant resilience and cannabinoid yields. When salt-stressed plants received both bacteria and biochar, shoot weight increased by 25%, root development improved by up to 38%, and crucially, CBD content rose by 13% while THC increased by 16% compared to stressed plants with no amendment. The plants also showed 49% higher essential oil yield and significantly reduced cellular damage (MDA levels dropped by 23%). These improvements came from enhanced nutrient availability and stress tolerance, with the bacteria mobilizing locked-up phosphorus while the biochar improved soil structure and water retention.

While this research was conducted in controlled greenhouse settings and requires field testing, the findings suggest a practical agricultural solution for farming cannabis in marginal, salt-affected soils—a significant problem in many regions. This integrated approach could allow cultivators to improve crop productivity and cannabinoid potency without relying solely on chemical fertilizers or abandoning saline-prone farmland, offering both economic and environmental benefits to the cannabis industry.

📄 Original Abstract

Soil salinity induces phosphorus (P) deficiency, limiting crop productivity, yet the combined use of phosphorus-solubilizing bacteria (PSB) and phosphorus-enriched biochar (P-biochar) under salinity remains unexplored. This study presents a novel integrated PSB+P-biochar approach to improve growth and secondary metabolite production in Cannabis sativa L. under salinity stress. A completely randomized design with three independent replicates (each replicate containing two pots) was employed. A factorial experiment was conducted with two factors: salinity at two levels (0 and 100mM NaCl) and soil amendment at six levels (control, B. subtilis, P. putida, P-biochar, B. subtilis + P-biochar, and P. putida + P-biochar). Seeds were inoculated with PSB and P-biochar was mixed into soil at 2% (w/w). Salinity decreased plant biomass, chlorophyll (Chl), relative water content (RWC), essential oil (EO) yield, and cannabinoids, while increasing proline and malondialdehyde (MDA) compared to the control (no salinity). In plants exposed to salinity stress, P-biochar in combination with B. subtilis and P. putida respectively increased shoot weight (25% and 24%), root weight (28% and 38%), Chl (33% and 29%), RWC (16% and 15%), proline (17% and 19%), EO yield (49% and 37%), cannabidiol (13% and 12%), and tetrahydrocannabinol (16% and 14%), but lowered MDA (23% and 21%) compared to salinity-only treatment. The results are limited to greenhouse conditions and require field validation. Nonetheless, this combined strategy is recommended to enhance plant resilience and optimize pharmacologically active compound production in saline environments.

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