What topics does this cannabis study cover?

This study covers research on: THC, CBD.

Where was this research published?

Published in Magnetic resonance in chemistry : MRC on July 23, 2026.

Faster, simpler testing for the cannabinoids in cannabis extracts

Q: What did this research study find?

An *ERETIC-based 1H-NMR* method simultaneously quantified acidic and neutral cannabinoids, including *THCA*, *CBDA*, *THC*, *CBD*, and *CBN*, in complex cannabis extracts.

Validation of ERETIC-Based 1H-NMR for the Simultaneous Quantification of Cannabinoids in Complex Cannabis sativa Extracts.

Magnetic resonance in chemistry : MRC • July 23, 2026 • Highly Relevant

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AI Summary

This study validated an ERETIC-based 1H-NMR method for measuring several cannabinoids at the same time in complex Cannabis sativa extracts. The approach quantified acidic and neutral forms, including THCA, CBDA, THC, CBD, and CBN, using diagnostic signals in spectral regions with minimal interference.

The method showed high precision (RSD <5%), 97%–102% recovery, and a minimum detection level of 0.07 mg/mL. Unlike traditional calibration, the ERETIC strategy does not require physical reference standards while producing equivalent results. For the cannabis industry and testing laboratories, this could support faster, reproducible profiling of extracts and classification by THC:CBD ratio, without derivatization.

💡 Key Findings

An ERETIC-based 1H-NMR method simultaneously quantified acidic and neutral cannabinoids, including THCA, CBDA, THC, CBD, and CBN, in complex cannabis extracts.

High

80%

The method demonstrated precision below 5% RSD, 97%–102% recovery, and a minimum detection level of 0.07 mg/mL.

High

80%

ERETIC calibration performed equivalently to traditional calibration while eliminating the need for physical reference standards, offering a rapid and reproducible alternative for cannabinoid analysis.

High

80%

The method successfully classified extracts by THC:CBD ratios, supporting practical chemotypic analysis.

Good

70%

📄 Original Abstract

Chromatographic methods for cannabinoid analysis currently face limitations, including lengthy procedures, artifact formation, and reliance on reference standards. This study establishes a robust quantitative 1H-NMR methodology for simultaneous analysis of acidic and neutral cannabinoids (THCA, CBDA, Δ9-THC, CBD, and CBN) in Cannabis sativa extracts. Two validated strategies, internal standard (butylated hydroxytoluene [BHT]) and ERETIC calibration, were compared, with diagnostic signals identified in interference-free spectral regions. The method demonstrated high precision (RSD < 5%), accuracy (recoveries 97%-102%), and low detection limits (MLOD 0.07 mg/mL). ERETIC outperformed traditional calibration by eliminating physical standards while maintaining equivalency. Successful chemotypic classification (THC:CBD ratios) confirmed practical applicability. The ERETIC-based NMR approach provides a rapid and reproducible alternative for comprehensive cannabinoid quantification without derivatization.

Paper Type

📄 Research

Publication

Journal: Magnetic resonance in chemistry : MRC
Published: July 23, 2026
PMID: 42487553
DOI: 10.1002/mrc.70135

Authors (4)

Rocío Figueroa Departamento de Química Orgánica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
Federico Arrighi Laboratorio de Investigaciones y Servicios Analíticos (LISA), Universidad Nacional de Tucumán-CONICET NOA Sur, San Miguel de Tucumán, Tucumán, Argentina.
Mauricio Cattaneo Laboratorio de Investigaciones y Servicios Analíticos (LISA), Universidad Nacional de Tucumán-CONICET NOA Sur, San Miguel de Tucumán, Tucumán, Argentina.
Miguel Gilabert Departamento de Química Orgánica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.