What topics does this cannabis study cover?

This study covers research on: THC, CBD, Pain, Inflammation, Endocannabinoid.

Where was this research published?

Published in Journal of endodontics on September 10, 2026.

THC CBD Pain Inflammation Endocannabinoid

CBD-THC combination shows promise in lab-grown dental pulp cells

Q: What did this research study find?

In human dental pulp cells exposed to *LPS*, the *CBD*/*THC* combination **more effectively reduced several pro-inflammatory cytokines than either treatment alone**.

Combined Cannabidiol and Δ9-Tetrahydrocannabinol Modulate Inflammation and Nociceptive Signaling in Human Dental Pulp Cells.

Journal of endodontics • September 10, 2026 • Highly Relevant

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AI Summary

This in vitro study asked whether combining CBD and THC could reduce inflammation and pain-related signaling while supporting repair in human dental pulp cells. Researchers exposed the cells to bacterial inflammation stimulus LPS and treated them with CBD (2.5 μg/mL), THC (0.6 μg/mL), or both. They measured inflammatory and anti-inflammatory signals, cannabinoid and pain-related markers, cell viability, proliferation, and migration. The abstract does not report the number of cell samples or treatment duration.

Compared with either cannabinoid alone, the combination more effectively reduced the pro-inflammatory cytokines IL-6, IL-12, and IL-1β, increased the anti-inflammatory cytokine IL-10, and altered markers linked to nociceptive signaling, including CNR1, CNR2, and TRPV1. It also enhanced cell proliferation and migration and increased markers associated with mineral formation, odontogenic differentiation, and angiogenesis. These results provide in vitro evidence that combined CBD and THC may influence processes involved in dental pulp repair, but this abstract-based summary cannot establish pain relief, safety, or treatment effectiveness in patients.

💡 Key Findings

In human dental pulp cells exposed to LPS, the CBD/THC combination more effectively reduced several pro-inflammatory cytokines than either treatment alone.

Moderate

55%

The combination increased IL-10 and modulated cannabinoid and nociceptive markers, including CNR1, CNR2, and TRPV1.

Moderate

55%

Combined CBD and THC enhanced cell proliferation and migration and increased markers linked to dental pulp repair, odontogenic differentiation, and angiogenesis.

Moderate

55%

The study identified non-cytotoxic concentrations for the tested cell treatment conditions, but the abstract provides no detailed safety results beyond the viability assessment.

Moderate

50%

📄 Original Abstract

INTRODUCTION: Dental pulp inflammation, caused by caries, trauma, or restorative operations, produces pain and slows regeneration. Cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC), cannabinoids of Cannabis sativa, have effects on inflammation and pain relief. However, the impact of these factors on dental pulp healing is not understood. This study investigated the combined effects of CBD and THC in controlling inflammation and nociception during dental pulp repair. METHODS: Human dental pulp cells were stimulated with lipopolysaccharide (LPS) and treated with CBD (2.5 μg/mL), THC (0.6 μg/mL), and their combination. Pro-inflammatory cytokines (IL-6, IL-12 and IL-1β) and anti-inflammatory cytokine IL-10 were analysed using qPCR and ELISA, while anti-nociceptive markers (CNR1, CNR2, and TRPV1) were evaluated by qPCR and Western blot. Cell viability, proliferation, and migration assays were performed to evaluate treatment effects on cellular behaviours associated with pulpal repair and regenerative potential. RESULTS: Compared with either treatment alone, the CBD/THC combination more effectively attenuated pro-inflammatory cytokines IL-6, IL-12, and IL-1β, enhanced CNR1 and CNR2 expression, modulated TRPV1, and increased IL-10 expression. Viability assays determined optimal non-cytotoxic concentrations for treatment. The combination enhanced cell proliferation and migration, promoted mineralized nodule formation, and significantly upregulated markers associated with tissue repair, odontogenic differentiation (DMP1), and angiogenesis (VEGF and bFGF). CONCLUSION: Combined CBD and THC show promise for promoting dental pulp healing by modulating inflammation and nociceptive signaling, while supporting odontogenic differentiation, angiogenesis, and cell migration in human dental pulp cells. These findings provide in vitro evidence supporting combined cannabinoid treatment as a therapeutic strategy for vital pulp therapies.

Paper Type

📄 Research

Publication

Journal: Journal of endodontics
Published: September 10, 2026
PMID: 42722230
DOI: 10.1016/j.joen.2026.09.003

Authors (13)

Zar Chi Soe Office of Research Affairs, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Daneeya Na Nan Office of Research Affairs, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Rahman Wahyudi Graduate Program in Oral Biology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Kanokwan Sriwattanapong Oral Biology Research Center, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand; Center of Excellence in Precision Medicine and Digital Health, Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Thantrira Porntaveetus Center of Excellence in Precision Medicine and Digital Health, Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand; Geriatric Dentistry and Special Patients Care International Program, Chulalongkorn University, Implant and Esthetic Center, Chulalongkorn University, Bangkok, Thailand; Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
+8 more authors